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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be observed in several tumors, which leads to chromosome instability, and thus may well lead to tumorigenesis. This protein was discovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and plays a significant role in advertising and marketing cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are actually described. Two further splice variants had been claimed but thorough nucleotide sequence facts is not nevertheless accessible.
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Blocking peptides are peptides that bind particularly towards the focus on antibody and block antibody binding. These peptide typically has the epitope recognized via the antibody.
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This guideline outlines even further attainable optimization of cellular and immunoassay parameters to ensure the best possible outcomes are...
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This information outlines further more possible optimization of cellular and immunoassay parameters to make certain the absolute best results are...
Antibodies certain to the blocking peptide no more bind into the epitope within the concentrate on protein. By comparing the staining through the blocked antibody compared to the antibody by itself, one can see which staining is distinct.
Focus on Data Cyclin E belongs to your remarkably conserved cyclin relatives, whose users are characterized by a extraordinary periodicity in protein abundance through the mobile cycle. Cyclins functionality as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add for the temporal coordination of every mitotic event. Cyclin E forms a posh with and functions as being a regulatory subunit of CDK2, whose action is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S section boundary and is also degraded as cells progress by S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by the two antibodies...
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